1. To complete the sequencing and assembly of the hard clam genome and develop an efficient and cost-effective genotyping platform (SNP array) for M. mercenaria.

1. Combine various technologies to sequence and assemble the hard clam genome, and establish a database of genetic variants (single nucleotide polymorphism or SNP) for the species 2. Build a SNP array and perform genomic selection for clams resistant to QPX disease and to heat stress 3. Establish clam breeding programs and provide the aquaculture industry with superior clam stocks

The hard clam, Mercenaria mercenaria, is among the most economically valuable bivalve species in the United States, representing the most important marine resource in several Atlantic states including New York. The species is extensively aquacultured from Massachusetts to Florida. Maintenance and growth of this aquaculture industry relies on hatchery production of seed, and billions of seed clams are produced annually to fulfill aquaculture and restoration needs. In this context, the production of quality seed able to survive under harsh biological and environmental conditions represents a major priority for the aquaculture community. This collaborative proposal builds on ongoing cooperation and new partnerships among Sea Grant programs, scientists and extension teams in 5 Atlantic states to develop a hard clam selective breeding program using state of the art genomic tools, for the benefit of clam farmers throughout the region.